Analysis Note

Negative ControlFisher rat embryo cells

Positive ControlB104-1-1 cells

Application

Frozen Sections (5 g/ml, see application references)Immunoblotting (see application references)Immunocytochemistry (5 g/ml)Immunoprecipitation (1 g/ml, see application references)Neutralization Studies (not recommended, see application references)

General description

Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay.

Purified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.

Immunogen

Epitope: Within the extracellular domain

B104-1-1 cells overexpressing rat c-ErbB2/c-Neu

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.

Zhang, H., et al. 1999. Exp. Mol. Pathol.67, 15.Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol.17, 1031.Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA85, 5389.Van De Vijver, M.J., et al. 1988. New Engl. J. Med.319, 1239.DiFiore, P.P., et al. 1987. Science237, 178.Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA84, 8498.Slamon, D.J., et al. Science235, 177.Varley, J.M., et al. 1987. Oncogene1, 423.Bargmann, C.I., et al. 1986. Nature319, 226.Yamamoto, T., et al. 1986. Nature319, 230.Blick, M., et al. 1984. Blood64, 1234.Schwab, M., et al. 1984. Cold Spring Harbor Laboratory2, 215.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)